ABSTRACT
OBJECTIVE To study the impr ovement effects of Dianxianqing granule on blood-brain barrier （BBB）injury in Alzheimer’s disease （AD）model mice by regulating NLR family pyrin domain containing 3（NLRP3）inflammasome signaling pathway. METHODS Totally 125 mice were randomly divided into sham operation group （n＝25）and modeling group （n＝100） by body weight. AD model was induced by intracerebroventricular injection of β-amyloid 25-35 in model group. Sham operation group was given normal saline with same method. The 100 model mice were randomly divided into model group ，Donepezil hydrochloride tablets group （positive control 1，1.3 mg/kg，i.g.），MCC950 group [positive control 2（selective NLRP 3 inhibitor），10 mg/kg，i.p.] and Dianxianqing granule group （12.48 g/kg by crude drug ，i.g.）by body weight ，with 25 mice in each group. Second day after modeling ，administration groups were given relevant medicine ，once a day ，for consecutive 21 d. Sham operation group and model group were given intragastric administration of water and intraperitoneal injection of normal saline. At last administration，the learning and memory ability was determined by Y maze test ，and blood-brain barrier permeability was measured by Evans blue leakage assay. The expressions of NLRP 3，anti-ionized calcium-binding adapter molecule 1（IBA-1），nuclear factor kappa B （NF-κB）p65，p53 upregulated modulator of apoptosis （PUMA），occludin（ocln），zonula occluden- 1（ZO-1）and claudin-5 （cldn5） in cerebral tissue were determined. RESULTS Compared with model group ， spontaneous alternate response rate ，protein expressions of ocln ，cldn5 lnzyxyqy2003@163.com and ZO- 1 in cerebral tissue were increased significantly in administration groups （P＜0.05 or P＜0.01）；Evans blue E-mail：jiadg2003@126.com content and protein expressions of NLRP 3，IBA-1，PUMA and NF-κB p65 in cerebral tissue were decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS Dianxianqing granule can improve BBB injury of AD model m ice by inhibiting NLRP 3 inflammasome signaling pathway.
ABSTRACT
OBJECTIVE@#To evaluate the feature and difference between treadmill exercise test (TET) and myocardial perfusion imaging (MPI) in myocardial bridge patients with symptoms. @*METHODS@#The data from 97 patients, who underwent 256-slice CT coronary angiography (CTCA) on TET and MPI, were collected and analyzed retrospectively. The patients were divided into an incomplete MB group (n=41) and a complete MB group (n=56). They were also divided into three groups according to the narrow degree of mural coronary artery (MCA) during the cardiac systolic period (Nobel grade): a Nobel 1 grade group (n=44), a Nobel 2 grade group (n=42), and a Nobel 3 grade group (n=11). Besides, the abnormal TET and MPI patients were divided into a TET abnormal group (n=21) and a MPI abnormal group (n=31). The results of TET and MPI were compared among the total, the different MB type groups and the different Noble grade groups, while the complete MB thickness and length were also compared between the abnormal TET group and the MPI abnormal group. @*RESULTS@#The positive rate of MPI (39/97) was higher than that of TET (26/97) in total (χ2=8.048, P0.05). There was a significant difference in the positive rate of TET (21/56) and MPI (31/56) in the complete MB group (χ2=8.333, P0.05). The complete MB thickness was (3.4±0.6) and (2.9±0.8) mm between the TET abnormal group and the MPI abnormal group, with statistically difference (t=2.229, P0.05). @*CONCLUSION@#MPI is more sensitive than TET in myocardial ischemia in patients with MB, especially to complete MB and mild-to-moderate systolic narrowing of MCA. The positive rate is low to incomplete MB, and it is highly sensitive to severe systolic narrowing of MCA. There is difference in the MB thickness between the two methods, but there is no difference in the MB length between the two methods.